MICROSCOPES

 

STEPS FOR USING A COMPOUND LIGHT MICROSCOPE:

 

1. Plug the microscope in, turn on the light and place the slide on the stage. Stage clips are optional.

 

2. Start with the scan objective (the shortest objective). The magnification is 4 X 10 = 40X. (Magnification is determined by the objective X the eyepiece.)

 

3. Adjust the diaphragm so the most light is allowed through. (#5)

 

4. Using the coarse adjustment knob (the biggest), slowly focus so that the stage moves AWAY from the objective until you just see image. You must do this step VERY SLOWLY.

 

5. Carefully move the slide until the image is in the center of the field of view.

NOTE: Moving the slide left will move the image right and moving the slide up will move the image down etc…

 

6. Using the fine adjustment knob, (the smaller knob) bring the image into focus.

 

7. Without touching the adjustment knobs, carefully switch to the low power objective (the medium sized one). You should look to the side while switching objectives. Magnification: ____________________.

NOTE: YOUR IMAGE SHOULD BE IN THE FIELD OF VIEW. IF NOT, START AGAIN AT THE BEGINNING.

 

8. You should only need to do some fine adjusting to bring the image into focus. The microscope has parfocal cabability which allows an image in focus at scan power to be in focus at low and high power.

 

9. Carefully move the slide until the image in the center of the field of view.

 

10. Without touching the adjustment knobs, carefully switch to the high power objective (the longest one). Look to the side again. Magnification: ____________________________

 

11. You should only need to do some fine adjusting to bring the image into focus. WARNING: NEVER USE THE COARSE ADJUSTMENT KNOB AT HIGH POWER.